Ham's Medium

Ham's Medium refers to a series of nutrient formulations originally developed by Dr. Richard Ham for the culture of various mammalian cells. Among these, Ham's F-12 Nutrient Mixture is a commonly used formulation known for its balanced composition that supports the growth of a wide range of cell types, especially those that require a more complex nutrient environment than offered by simpler media.

Technical Composition

Ham's F-12 Medium is characterized by a rich blend of nutrients, salts, amino acids, vitamins, and trace elements. Its formulation includes:

  • Trace Elements and Minerals:
    • Copper, zinc, iron, manganese, and selenium often included at low concentrations which serve as essential cofactors for enzymatic reactions.
  • Glucose and Other Energy Sources:
    • Glucose is typically included as a primary carbon and energy source. Some formulations may also include other metabolites to support energy-intensive processes.
  • Buffering System:
    • Sodium bicarbonate is used to stabilize pH in a CO₂ atmosphere. In some protocols, additional buffers like HEPES might be added to maintain pH stability under non-CO₂ conditions.
Applications in Cell Culture

Ham's F-12 Medium is particularly suited for cells that have more stringent nutritional or metabolic requirements. Its applications include:

  • Support of Diverse Cell Lines:
    • Suitable for a variety of cell lines including epithelial cells, fibroblasts, and certain stem cells that thrive on a nutrient-rich environment.
  • Specialized Differentiation Studies:
    • The complex nutrient composition supports differentiation protocols where a more comprehensive nutrient profile can influence cell fate decisions.
  • Serum-Free and Defined Culture Systems:
    • Often used as a base for serum-free or chemically defined culture media formulations, where its rich mixture of nutrients helps to reduce or eliminate the need for animal serum supplementation.

By offering a comprehensive array of nutrients, Ham's Medium—particularly Ham's F-12—provides a versatile foundation for cultivating a diverse range of mammalian cells. Its detailed formulation supports complex cellular processes, making it an invaluable tool for both routine culture and specialized experimental applications.

131.25 131.25 USD
131.25 131.25 USD
116.25 116.25 USD
116.25 116.25 USD
116.25 116.25 USD
116.25 116.25 USD
AffiMEDIUM® Ham's F-10 Complete Medium (20%FBS)
CAT# AFG-LBM-0822
Size: 125 mL×4
216.00 216.0 USD
AffiMEDIUM® Ham's F-10 Complete Medium (10%FBS)
CAT# AFG-LBM-0821
Size: 125 mL×4
216.00 216.0 USD
AffiMEDIUM® Ham's F-12K Complete Medium (20%FBS)
CAT# AFG-LBM-0819
Size: 125 mL×4
171.00 171.0 USD
AffiMEDIUM® Ham's F-12 Complete Medium (20%FBS)
CAT# AFG-LBM-0818
Size: 125 mL×4
216.00 216.0 USD
AffiMEDIUM® Ham's F-12 Complete Medium (10%FBS)
CAT# AFG-LBM-0817
Size: 125 mL×4
171.00 171.0 USD
AffiMEDIUM® Ham's F-12K (without L-glutamine)
CAT# AFG-LBM-0770
Size: 500 mL
105.00 105.0 USD
AffiMEDIUM® Ham's F-12K (with PS)
CAT# AFG-LBM-0769
Size: 500 mL
116.25 116.25 USD
AffiMEDIUM® Ham's F-12 (without phenol red)
CAT# AFG-LBM-0767
Size: 500 mL
105.00 105.0 USD
AffiMEDIUM® Ham's F-12 (without L-glutamine)
CAT# AFG-LBM-0766
Size: 500 mL
120.00 120.0 USD
AffiMEDIUM® Ham's F-12 (with PS)
CAT# AFG-LBM-0765
Size: 500 mL
112.50 112.5 USD
AffiMEDIUM® Ham's F-10 (without phenol red)
CAT# AFG-LBM-0764
Size: 500 mL
112.50 112.5 USD
AffiMEDIUM® Ham's F-10 (without L-glutamine)
CAT# AFG-LBM-0763
Size: 500 mL
112.50 112.5 USD
AffiMEDIUM® Ham's F-10 (with PS)
CAT# AFG-LBM-0760
Size: 500 mL
112.50 112.5 USD
146.25 146.25 USD
146.25 146.25 USD
131.25 131.25 USD
146.25 146.25 USD
116.25 116.25 USD
116.25 116.25 USD
AffiMEDIUM® Ham's F-12K Complete Medium (10%FBS)
CAT# AFG-LBM-0182
Size: 125 mL×4
171.00 171.0 USD
AffiMEDIUM® Ham's F-12K (without phenol red)
CAT# AFG-LBM-0100
Size: 500 mL
120.00 120.0 USD
112.50 112.5 USD
AffiMEDIUM® Ham's F-12K
CAT# AFG-LBM-0098
Size: 500 mL
97.50 97.5 USD
105.00 105.0 USD
AffiMEDIUM® Ham's F-12
CAT# AFG-LBM-0096
Size: 500 mL
97.50 97.5 USD
112.50 112.5 USD
AffiMEDIUM® Ham's F-10
CAT# AFG-LBM-0094
Size: 500 mL
108.75 108.75 USD
AffiMEDIUM® Ham's F-12K, powder
CAT# AFG-LBM-0011
Size: 5×1 L
116.25 116.25 USD
AffiMEDIUM® Ham's F-12, powder
CAT# AFG-LBM-0010
Size: 5×1 L
116.25 116.25 USD
162.00 162.0 USD
162.00 162.0 USD