IMDM Medium
Iscove's Modified Dulbecco's Medium (IMDM) is a highly enriched cell culture medium, designed for the cultivation of a broad range of mammalian cells, particularly hematopoietic cells and hybridomas. It is a modification of Dulbecco's Modified Eagle Medium (DMEM) and was developed by Dr. Norman Iscove to support the growth of cells in serum-free or low-serum conditions.
Key Components
- Amino Acids: IMDM contains both essential and non-essential amino acids, including higher concentrations of L-glutamine and L-arginine compared to DMEM, supporting protein synthesis and cell growth.
- Vitamins: The medium is supplemented with an extensive array of vitamins, including folic acid, inositol, and ascorbic acid, which are essential for cell metabolism and proliferation.
- Glucose: IMDM typically contains a lower glucose concentration (approximately 4.5 g/L), providing an energy source for cell metabolism while reducing the risks of excessive lactate production.
- Inorganic Salts: These salts, including calcium chloride, magnesium sulfate, and potassium nitrate, maintain osmotic balance, membrane potential, and provide necessary ions for enzymatic functions.
- Sodium Pyruvate: Acts as an additional carbon source, supporting the tricarboxylic acid (TCA) cycle and cellular respiration.
- Hemin: An iron-containing compound included to support the growth of hematopoietic cells, providing a source of heme for hemoglobin synthesis.
- Phenol Red: A pH indicator included in the medium to monitor pH changes during cell culture.
Applications
- Hematopoietic Cell Culture: IMDM is especially suitable for culturing lymphocytes, macrophages, and other cells derived from bone marrow or blood.
- Hybridoma Culture: It supports the growth and maintenance of hybridoma cells, which are used in the production of monoclonal antibodies.
- Serum-Free Culture: IMDM is often used in serum-free conditions with appropriate supplements, making it ideal for experiments where serum components might interfere with results.
- Genetic Studies: The medium is commonly used in transfection experiments and genetic modification studies due to its robust support of cell viability and proliferation.
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