IMDM Medium
IMDM (Iscove’s Modified Dulbecco’s Medium) is a widely used synthetic medium developed for the cultivation of mammalian cells. It has been optimized to support high-density cell growth and facilitate the production of various cellular products such as proteins, monoclonal antibodies, and other biopharmaceuticals.
Content of IMDM Medium
The IMDM Medium AffiMEDIUM contains the following critical components:
- Amino Acids
- Essential and non-essential amino acids support protein synthesis and cellular growth.
- Vitamins
- A complete range of vitamins (e.g., B-complex, vitamin C) ensures proper enzymatic function and metabolic support.
- Inorganic Salts
- Includes salts such as NaCl, KCl, CaCl₂, and MgSO₄ to maintain osmotic balance and provide necessary ions for cellular activity.
- Energy Sources
- Glucose and other carbohydrates are included as primary energy sources for cellular metabolism.
Application of IMDM Medium
Cell Culture and Expansion
- Ideal for culturing lymphocytes, hybridomas, and other fastidious mammalian cell types. It ensures optimal growth rates and high cell viability.
Monoclonal Antibody Production
- The medium supports the expression and secretion of antibodies in hybridoma or transfected cell lines.
Affinity Chromatography Integration
- AffiMEDIUM is compatible with affinity purification processes, reducing interference from medium components during downstream protein purification.
IMDM Medium AffiMEDIUM is a robust tool for biopharmaceutical manufacturing and research, enabling scalable and efficient production of biologics. Its compatibility with both upstream and downstream processes makes it a preferred choice for advanced cell culture applications.
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