IMDM Medium

Iscove's Modified Dulbecco's Medium (IMDM) is a highly enriched cell culture medium, designed for the cultivation of a broad range of mammalian cells, particularly hematopoietic cells and hybridomas. It is a modification of Dulbecco's Modified Eagle Medium (DMEM) and was developed by Dr. Norman Iscove to support the growth of cells in serum-free or low-serum conditions.

Key Components
  • Amino Acids: IMDM contains both essential and non-essential amino acids, including higher concentrations of L-glutamine and L-arginine compared to DMEM, supporting protein synthesis and cell growth.
  • Vitamins: The medium is supplemented with an extensive array of vitamins, including folic acid, inositol, and ascorbic acid, which are essential for cell metabolism and proliferation.
  • Glucose: IMDM typically contains a lower glucose concentration (approximately 4.5 g/L), providing an energy source for cell metabolism while reducing the risks of excessive lactate production.
  • Inorganic Salts: These salts, including calcium chloride, magnesium sulfate, and potassium nitrate, maintain osmotic balance, membrane potential, and provide necessary ions for enzymatic functions.
  • Sodium Pyruvate: Acts as an additional carbon source, supporting the tricarboxylic acid (TCA) cycle and cellular respiration.
  • Hemin: An iron-containing compound included to support the growth of hematopoietic cells, providing a source of heme for hemoglobin synthesis.
  • Phenol Red: A pH indicator included in the medium to monitor pH changes during cell culture.
Applications
  • Hematopoietic Cell Culture: IMDM is especially suitable for culturing lymphocytes, macrophages, and other cells derived from bone marrow or blood.
  • Hybridoma Culture: It supports the growth and maintenance of hybridoma cells, which are used in the production of monoclonal antibodies.
  • Serum-Free Culture: IMDM is often used in serum-free conditions with appropriate supplements, making it ideal for experiments where serum components might interfere with results.
  • Genetic Studies: The medium is commonly used in transfection experiments and genetic modification studies due to its robust support of cell viability and proliferation.

AffiMEDIUM® IMDM Complete Medium (20%FBS)
CAT# AFG-LBM-0811
Size: 125 mL×4
216.00 216.0 USD
AffiMEDIUM® IMDM Complete Medium (10%FBS)
CAT# AFG-LBM-0810
Size: 125 mL×4
171.00 171.0 USD
AffiMEDIUM® IMDM (glucose free)
CAT# AFG-LBM-0773
Size: 500 mL
105.00 105.0 USD
112.50 112.5 USD
AffiMEDIUM® IMDM (with PS)
CAT# AFG-LBM-0771
Size: 500 mL
112.50 112.5 USD
146.25 146.25 USD
131.25 131.25 USD
AffiMEDIUM® Nanobacteria Removal Medium (IMDM)
CAT# AFG-LBM-0569
Size: 100 mL
116.25 116.25 USD
AffiMEDIUM® Mycoplasma Removal Medium (IMDM)
CAT# AFG-LBM-0563
Size: 100 mL
116.25 116.25 USD
120.00 120.0 USD
AffiMEDIUM® IMDM (without phenol red)
CAT# AFG-LBM-0104
Size: 500 mL
116.25 116.25 USD
AffiMEDIUM® IMDM (with L- alanyl -L-glutamine)
CAT# AFG-LBM-0103
Size: 500 mL
105.00 105.0 USD
AffiMEDIUM® IMDM (without L-glutamine)
CAT# AFG-LBM-0102
Size: 500 mL
105.00 105.0 USD
AffiMEDIUM® IMDM
CAT# AFG-LBM-0101
Size: 500 mL
97.50 97.5 USD
AffiMEDIUM® IMDM, powder
CAT# AFG-LBM-0007
Size: 5×1 L
116.25 116.25 USD