Content of McCoy's Medium
McCoy's Medium is a type of cell culture medium developed initially for the culture of Novikoff hepatoma cells. It has since been adapted for a variety of mammalian cell types. Here is a brief technical overview:
Composition:
- Amino Acids: Essential and non-essential amino acids to support protein synthesis.
- Vitamins: A range of vitamins to support cellular metabolism.
- Salts: Inorganic salts to maintain osmotic balance and provide essential ions.
- Glucose: Provides a primary energy source for cell metabolism.
- Phenol Red: A pH indicator to monitor the medium's pH changes.
- L-Glutamine: An amino acid that serves as a nitrogen source for cells.
Function:
- Cell Growth: Supports the growth of a wide variety of mammalian cells.
- Maintenance: Used for maintaining cell lines over extended periods.
- Research Applications: Commonly used in cancer research, virology, and cytogenetic studies.
Preparation and Use:
- Typically supplied as a liquid or powder.
- Requires sterile filtration before use to ensure the absence of contaminants.
- Supplementation with serum (such as fetal bovine serum) is often necessary to provide additional growth factors.
Technical Details:
- pH Range: 7.0-7.4 to mimic physiological conditions.
- Osmolality: Around 290-310 mOsm/kg to maintain cell membrane integrity and function.
- Sterilization: Sterilized through filtration to maintain a sterile environment for cell culture.
Applications:
- Cancer Research: Frequently used to culture cancer cell lines for research purposes.
- Virology: Supports the growth of cells used in virus cultivation and studies.
- Toxicology: Employed in testing the effects of drugs and toxins on mammalian cells.
- Cytogenetics: Used in genetic studies involving the growth and analysis of chromosomes.
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|