Mycoplama Removal Medium

Mycoplasma Removal Medium (MRM) is a specialized cell culture medium designed to eliminate mycoplasma contamination in mammalian cell cultures. Mycoplasma contamination is a common and significant issue in cell culture laboratories, as it can interfere with cellular processes, alter experimental results, and reduce cell viability. MRM combines selective anti-mycoplasma agents with nutrients to maintain cell growth while effectively eradicating mycoplasma.

Composition of Mycoplasma Removal Medium

• Selective Anti-Mycoplasma Agents
  • Includes compounds such as antibiotics (e.g., quinolones, tetracyclines) or specific mycoplasma inhibitors that target mycoplasma DNA replication and protein synthesis without harming mammalian cells.
• Essential Nutrients
  • A complete set of amino acids, vitamins, and carbohydrates supports cell survival and recovery during treatment.
• Buffering System
  • Typically contains sodium bicarbonate or HEPES to maintain pH stability in CO₂-incubated or CO₂-free conditions.
• Supplementary Components
  • Optional growth factors, lipids, or protective agents (e.g., antioxidants) to minimize cellular stress during the treatment.

Applications of Mycoplasma Removal Medium

Mycoplasma Decontamination

• Used to eradicate mycoplasma from contaminated mammalian cell cultures, including adherent and suspension cells.

Cell Line Recovery

• Helps restore functionality and viability of cell lines impacted by mycoplasma, making them suitable for downstream applications.

Research and Industrial Laboratories

• Essential for maintaining contamination-free cell cultures in academic research, biotechnology, and pharmaceutical production.

Mycoplasma Removal Medium is a crucial tool for addressing one of the most persistent challenges in cell culture. By effectively eliminating mycoplasma contamination while preserving cell health, it ensures the integrity of experimental results and the reliability of cell-based production systems.