Nanobacteria Removal Medium
Nanobacteria Removal Medium (NRM) is a specialized culture medium developed to eliminate nanobacteria contamination from mammalian cell cultures. Nanobacteria, controversial submicroscopic entities often associated with calcification processes, can interfere with cell culture systems, leading to changes in cellular behavior, reduced growth, and experimental variability.
Composition of Nanobacteria Removal Medium
- Anti-Nanobacteria Agents
- Includes chelating agents (e.g., EDTA) to disrupt calcified structures associated with nanobacteria.
- Antibiotics targeting nanobacteria-like entities, such as tetracyclines or macrolides, which inhibit protein synthesis and cellular replication.
- Decalcifying Compounds
- Components like citrate or phosphonates to dissolve calcified deposits formed by nanobacteria.
- Cellular Support Nutrients
- A balanced mixture of amino acids, vitamins, and energy sources (e.g., glucose) to sustain mammalian cell growth during treatment.
- Buffering System
- Sodium bicarbonate or HEPES buffer ensures pH stability in a CO₂-regulated environment.
Applications of Nanobacteria Removal Medium
Nanobacteria Decontamination
- Specifically designed to remove nanobacteria from contaminated cell cultures, including adherent and suspension cell lines.
Calcium-Dependent Processes
- Applied in cultures with calcification-related issues that may arise due to nanobacteria contamination.
Research and Diagnostics
- Used in laboratories focusing on the biological implications of nanobacteria in cell cultures and their impact on experimental outcomes.
Routine Laboratory Use
- Preventive treatment for high-risk cell lines prone to contamination with calcifying agents.
Nanobacteria Removal Medium is an essential tool for laboratories working with calcification-prone cell cultures or exploring the role of nanobacteria in biological systems. By targeting both nanobacteria and their calcifications, NRM ensures healthier, contamination-free cultures, improving experimental reliability and cell line quality.
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