Rat Complete Medium
Rat Complete Medium" refers to a cell culture medium specifically formulated to support the growth and maintenance of rat-derived cells. Like other specialized media, Rat Complete Medium is designed to meet the particular needs of rat cells in culture, including their nutritional and environmental requirements.
Key Components
Basal Medium
- DMEM (Dulbecco's Modified Eagle Medium): Often used as a base, providing essential nutrients including amino acids, vitamins, glucose, and salts.
- RPMI-1640: Another commonly used basal medium that may be employed depending on the cell type and specific needs.
Serum
- Fetal Bovine Serum (FBS): Typically added at 5-20% concentration to supply growth factors, hormones, and additional nutrients crucial for cell proliferation and survival.
- Rat Serum: In some cases, rat serum may be used to more closely mimic the native environment of rat cells, though FBS is more common.
Supplements
- L-Glutamine: An essential amino acid often added to support cellular metabolism and protein synthesis.
- Antibiotics: Penicillin and streptomycin are commonly included to prevent bacterial contamination.
Buffering System
- Sodium Bicarbonate: Used in conjunction with CO₂ to maintain physiological pH in the medium.
- HEPES Buffer: An alternative buffering agent for maintaining pH stability in ambient air, useful when CO₂ incubation is not available.
Phenol Red: A pH indicator dye used to monitor changes in the medium's pH visually.
Applications
- Primary Cell Culture: Rat Complete Medium is used to culture primary cells isolated from rat tissues such as liver, kidney, heart, or neurons.
- Cell Line Maintenance: It supports established rat cell lines, including those derived from tissues like fibroblasts, epithelial cells, or cancerous tissues.
- Research: Utilized in various research areas including pharmacology, toxicology, and immunology, where rat models are relevant.
- Tissue Engineering: Supports the growth of rat cells in tissue engineering applications, helping to maintain their specific characteristics.
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