RPMI Medium

RPMI (Roswell Park Memorial Institute) medium is a widely used cell culture medium originally developed for the cultivation of human leukocytes. Its balanced formulation supports the growth of a broad range of cell types, including hybridomas, hematopoietic cells, and tumor cell lines. RPMI-1640, the most common variant, is favored for its versatility and adaptability.

Content

RPMI-1640 contains the following key components:

  • Inorganic Salts:
    • Sodium chloride, potassium chloride, calcium chloride, and magnesium sulfate maintain osmotic balance and provide essential ions.
  • Buffer System:
    • Sodium bicarbonate buffers the medium at physiological pH when used in a CO₂ incubator.
  • Amino Acids:
    • L-glutamine serves as an energy source and a precursor for nucleotide and protein synthesis.
  • Vitamins:
    • Essential vitamins like folic acid, riboflavin, niacinamide, and biotin support metabolic and enzymatic functions.

Applications

  • Immune Cell Culture:
    • Widely used for culturing lymphocytes, monocytes, and macrophages in immunological research.
  • Cancer Research:
    • Supports the growth of tumor cell lines, particularly hematopoietic and lymphoid cells, for studies on cancer biology and drug screening.
  • Hybridoma Technology:
    • Used in the generation of monoclonal antibodies, as it supports the growth of hybridoma cells.
  • Toxicity Testing:
    • Serves as a platform for evaluating the cytotoxicity of drugs, chemicals, and environmental toxins on various cell lines.

RPMI medium is a versatile and essential tool in cell biology research. Its balanced formulation provides an ideal environment for diverse cell types, making it a cornerstone in immunology, cancer research, and drug discovery. Proper supplementation and handling ensure consistent and reproducible results in various applications.

AffiMEDIUM® RPMI-1640 Complete Medium (20%FBS)
CAT# AFG-LBM-0798
Size: 500 mL
120.00 120.0 USD
AffiMEDIUM® RPMI-1640 (with MOPS)
CAT# AFG-LBM-0690
Size: 500 mL
138.75 138.75 USD
AffiMEDIUM® RPMI-1640 (CTL)
CAT# AFG-LBM-0689
Size: 500 mL
138.75 138.75 USD
AffiMEDIUM® SILAC RPMI-1640 Medium
CAT# AFG-LBM-0688
Size: 500 mL
138.75 138.75 USD
116.25 116.25 USD
116.25 116.25 USD
108.75 108.75 USD
AffiMEDIUM® RPMI-1640 (Dutch Modification)
CAT# AFG-LBM-0684
Size: 500 mL
112.50 112.5 USD
AffiMEDIUM® RPMI-1640 (2×)
CAT# AFG-LBM-0683
Size: 500 mL
112.50 112.5 USD
112.50 112.5 USD
108.75 108.75 USD
108.75 108.75 USD
112.50 112.5 USD
105.00 105.0 USD
101.25 101.25 USD
AffiMEDIUM® RPMI-1640 (with HEPES, PS)
CAT# AFG-LBM-0673
Size: 500 mL
116.25 116.25 USD
AffiMEDIUM® RPMI-1640 (with PS)
CAT# AFG-LBM-0672
Size: 500 mL
112.50 112.5 USD
146.25 146.25 USD
131.25 131.25 USD
116.25 116.25 USD
116.25 116.25 USD
AffiMEDIUM® RPMI 8226 Cells Complete Medium
CAT# AFG-LBM-0499
Size: 125 mL×4
163.50 163.5 USD
AffiMEDIUM® RPMI-1640 Complete Medium (10%FBS)
CAT# AFG-LBM-0175
Size: 125 mL×4
171.00 171.0 USD
AffiMEDIUM® RPMI-1640 (ATCC Modification)
CAT# AFG-LBM-0059
Size: 500 mL
116.25 116.25 USD
112.50 112.5 USD
112.50 112.5 USD
108.75 108.75 USD
AffiMEDIUM® RPMI-1640 (glucose free)
CAT# AFG-LBM-0054
Size: 500 mL
105.00 105.0 USD
105.00 105.0 USD
AffiMEDIUM® RPMI-1640 (without phenol red)
CAT# AFG-LBM-0052
Size: 500 mL
105.00 105.0 USD
108.75 108.75 USD
108.75 108.75 USD
105.00 105.0 USD
AffiMEDIUM® RPMI-1640 (without L-glutamine)
CAT# AFG-LBM-0048
Size: 500 mL
101.25 101.25 USD
AffiMEDIUM® RPMI-1640 (with HEPES)
CAT# AFG-LBM-0047
Size: 500 mL
101.25 101.25 USD
AffiMEDIUM® RPMI-1640
CAT# AFG-LBM-0046
Size: 500 mL
97.50 97.5 USD
AffiMEDIUM® RPMI-1640, powder
CAT# AFG-LBM-0001
Size: 5×1 L
112.50 112.5 USD