Cells Complete Medium

William’s E Medium is a specialized cell culture medium formulated to support the growth and maintenance of liver-derived cells, particularly hepatocytes. Developed for liver perfusion and primary liver cell culture, it is widely used in toxicology, metabolism, and pharmacological studies. The medium provides a balanced composition of nutrients, vitamins, and cofactors essential for liver-specific functions.

Content

William’s E Medium contains the following key components:

• Carbohydrate Source
  • D-glucose provides a readily available energy source for cellular metabolism.
• Trace Elements
  • Zinc sulfate and other trace elements are included to support enzymatic and structural functions in cells.
• Buffer System
  • Sodium bicarbonate ensures pH stability under a CO₂ atmosphere.
• Additional Supplements (optional)
  • Often supplemented with fetal bovine serum (FBS), insulin, transferrin, and dexamethasone to further support hepatocyte viability and liver-specific functions.

Applications

• Primary Hepatocyte Culture
  • Optimized for the isolation and maintenance of primary hepatocytes from human or animal sources.
• Drug Metabolism and Toxicity Studies
  • Used in ADME (Absorption, Distribution, Metabolism, and Excretion) studies to evaluate the effects of pharmaceuticals and xenobiotics on liver function.
• Liver Function Studies
  • Supports research on liver-specific processes such as albumin production, urea synthesis, and cytochrome P450 enzyme activity.
• 3D Liver Models
  • Serves as a medium for 3D culture systems, such as spheroids or organoids, to mimic liver tissue architecture.
• Tissue Engineering and Regenerative Medicine
  • Used in studies exploring liver regeneration and hepatocyte transplantation.

William’s E Medium is a critical tool for liver cell research, particularly in applications requiring hepatocyte viability and function. Its carefully balanced formulation provides the necessary nutrients to support liver-specific studies in pharmacology, toxicology, and cell biology. Proper handling and supplementation are essential for achieving reliable and reproducible results.