Overview of CTLA Medium

CTLA Medium is a type of cell culture medium designed to support the growth and maintenance of specific cell types, typically T lymphocytes, which are crucial components of the immune system. The medium provides essential nutrients, growth factors, and hormones required for the optimal proliferation and function of these cells.

Content and Composition

  • Basal Medium:
    • RPMI 1640: Often the base medium, which includes essential salts, glucose, amino acids, and vitamins.
  • Serum:
    • Fetal Bovine Serum (FBS): Provides additional growth factors, hormones, and nutrients. The concentration can vary but is typically around 10%.
  • Growth Factors and Cytokines:
    • Interleukin-2 (IL-2): A cytokine that promotes T cell proliferation.
    • CTLA-4 (Cytotoxic T-Lymphocyte-Associated Protein 4): Often used in contexts involving immune checkpoint studies.
  • Antibiotics and Antimycotics:
    • Penicillin-Streptomycin: To prevent bacterial contamination.
    • Amphotericin B: To prevent fungal contamination.
  • Buffer System:
    • HEPES Buffer: Maintains pH stability during cell culture.
  • Additional Supplements:
    • Glutamine: An essential amino acid that supports cell growth and metabolism.
    • Beta-mercaptoethanol: A reducing agent that can improve cell viability and growth.

Technical Aspects

  • pH and Osmolality: The medium is adjusted to physiological pH (around 7.2-7.4) and osmolality to mimic the natural extracellular environment of T lymphocytes.
  • Sterility: The medium must be sterile to prevent contamination and ensure reliable cell culture results.
  • Storage and Stability: Typically stored at 2-8°C. The stability can vary, with certain components like IL-2 being more sensitive to temperature and requiring proper handling.


  • T Cell Expansion: Used extensively in research involving T cell activation and proliferation.
  • Immunotherapy Research: Critical for culturing T cells for therapeutic purposes, such as CAR-T cell therapy.
  • Immunological Studies: Helps in studying T cell functions, interactions, and immune responses.
AffiMEDIUM® CTLA4 Ig-24 Cells Complete Medium
Size: 125 mL×4
103.10 103.10000000000001 USD
Size: 500 mL
142.50 142.5 USD