Overview of RFP Medium

RFP Medium is a specialized culture medium designed for the growth and maintenance of cells expressing Red Fluorescent Protein (RFP). This medium is optimized to support the stable expression of RFP in various cell lines, ensuring high levels of fluorescence for downstream applications.

Technical Content

Key Features:

• Optimized Formulation: The medium is precisely formulated to enhance the expression and stability of RFP in cultured cells.
• High Fluorescence Intensity: Supports robust fluorescence, making it ideal for imaging and fluorescence-based assays.
• Enhanced Cell Viability: Provides essential nutrients and growth factors that promote cell health and proliferation.

Applications:

• Cell Imaging: Ideal for fluorescence microscopy and live-cell imaging studies involving RFP-expressing cells.
• Fluorescence-Based Assays: Suitable for assays that rely on RFP signal detection, such as FACS analysis and reporter gene assays.
• Protein Expression Studies: Facilitates the study of gene expression and protein localization in RFP-expressing cells.

Usage Protocol:

• Preparation: Thaw the RFP Medium at 2-8°C. Mix thoroughly before use.
• Cell Culture: Seed cells expressing RFP into culture vessels containing RFP Medium. Maintain cells under standard culture conditions (e.g., 37°C, 5% CO2).
• Maintenance: Replace the medium regularly (e.g., every 2-3 days) to ensure optimal growth and fluorescence.
• Assay Performance: Perform imaging or fluorescence-based assays as required, ensuring minimal disturbance to the cells.

Benefits:

• Consistency: Provides a stable environment for maintaining consistent RFP expression levels.
• High Sensitivity: Ensures strong fluorescence signals, enhancing the sensitivity of detection methods.
• Convenience: Ready-to-use medium simplifies cell culture workflows and reduces preparation time.