Cells Complete Medium
Cells Complete Medium is a type of culture medium used in laboratories for the in vitro growth and maintenance of cell lines. It is designed to provide a nutrient-rich environment that supports cellular growth, proliferation, and viability. This medium typically contains a balanced mixture of essential components, including amino acids, vitamins, inorganic salts, glucose, and other supplements necessary for cellular metabolism.
Content of Cells Complete Medium
- Amino Acids:
- Essential for protein synthesis and cell growth.
- Includes both essential and non-essential amino acids.
- Vitamins:
- Required for enzymatic activities and cellular functions.
- Includes vitamins like B-complex (B1, B2, B6, B12), Vitamin C, and folic acid.
- Inorganic Salts:
- Maintain osmotic balance and provide ions for cellular activities.
- Common salts include sodium chloride (NaCl), potassium chloride (KCl), magnesium sulfate (MgSO4), and calcium chloride (CaCl2).
- Glucose:
- Primary energy source for cells.
- Supports cellular respiration and energy production.
- Serum:
- Often supplemented with fetal bovine serum (FBS) or other sera.
- Provides growth factors, hormones, and additional nutrients.
- Buffering Agents:
- Maintain pH stability in the medium.
- Common buffers include sodium bicarbonate (NaHCO3) and HEPES.
- Antibiotics and Antimycotics (optional):
- Prevent bacterial and fungal contamination.
- Includes agents like penicillin, streptomycin, and amphotericin B.
Usage and Applications
- Cell Line Maintenance: Used for routine culturing and sub-culturing of various cell lines, ensuring cells remain healthy and proliferative.
- Experimental Research: Provides a controlled environment for cellular experiments, including drug testing, genetic studies, and cellular responses to various stimuli.
- Biotechnological Applications: Essential for the production of biological products, such as monoclonal antibodies, recombinant proteins, and vaccines.
Advantages
- Supports robust cell growth and viability.
- Consistent and reproducible results due to defined composition.
- Versatile, suitable for a wide range of cell types and applications.
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